AICON-BIO makes lyophilized veterinary PCR kits — 50 real-time assays covering the bacterial, viral and parasitic diseases of cattle, sheep and goats, horses, pigs, poultry and companion animals. Every kit uses the same one-step TaqMan chemistry, freeze-dried into a single tube, so one workflow and one thermal programme run the whole range.

50Catalogue kits
7Species groups
48T / 96TPack sizes
~75 minSample to answer

The platform

One chemistry behind every veterinary PCR kit


Primers, probe, enzyme and dNTPs are already inside the tube. The laboratory adds reconstitution buffer and 5 µL of extracted nucleic acid, and runs one programme. The same instrument settings and the same interpretation rules apply across all 50 products, which is what makes species-level panels practical rather than theoretical.

AmbientNo cold chainLyophilized reactions tolerate the temperature swings of long-distance freight. No dry ice, no failed shipments.
2 stepsMinimal pipettingReconstitute, add template, run. Removing the master-mix step removes the commonest cause of a failed run.
OpenAny qPCR instrumentABI, Bio-Rad, Roche LightCycler, QIAGEN Rotor-Gene, Agilent and others. FAM channel only — no locked consumables.
IncludedControls in every boxA synthetic plasmid positive control and a negative control, so run validity is established on every plate.
MethodOne-step real-time PCR, TaqMan hydrolysis probe
Reagent formatLyophilized single-tube reactions, ambient-stable
Detection channelFAM
Reaction volume25 µL (20 µL reconstituted reagent + 5 µL template)
Cycling50 °C 10 min → 95 °C 3 min → 45 × (95 °C 15 s, 60 °C 30 s)
InterpretationCt < 37 positive · 37–45 retest · no Ct negative
Pack sizes48T and 96T
Shelf life12 months
OriginMade in USA — Aicon Biotech Inc, Tigard, Oregon

The range

Veterinary PCR kits by target animal


Cattle & ruminants

6 kits

Abortion investigation, tick-borne disease, tuberculosis and the notifiable diseases that govern herd movement and export certification.

AICON-BIO® Bluetongue Virus (BTV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Bluetongue Virus (BTV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting BTV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Brucella abortus Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Brucella abortus Real-time PCR Kit provides a fast, accurate and reliable method for detecting Brucella abortus nucleic acid using real-time PCR technology. Validated on whole blood, serum, tissue from cattle, other livestock. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Brucella spp. Real-time PCR Kit (Ruminant)

$338.00$672.00

The AICON-BIO® Brucella spp. Real-time PCR Kit (Ruminant) provides a fast, accurate and reliable method for detecting Brucella nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Lumpy Skin Disease Virus (LSDV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Lumpy Skin Disease Virus (LSDV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting LSDV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Theileria spp. Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Theileria spp. Real-time PCR Kit provides a fast, accurate and reliable method for detecting Theileria spp. nucleic acid using real-time PCR technology. Validated on whole blood, lymph node, spleen from cattle, sheep. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

Sheep & goats

3 kits

The respiratory and abortion pathogens of small ruminants, including the WOAH-listed diseases that control flock movement.

AICON-BIO® Brucella melitensis Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Brucella melitensis Real-time PCR Kit provides a fast, accurate and reliable method for detecting Brucella melitensis nucleic acid using real-time PCR technology. Validated on whole blood, serum, tissue from sheep, goats, other livestock. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Brucella species Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Brucella melitensis Real-time PCR Kit provides a fast, accurate and reliable method for detecting Brucella melitensis nucleic acid using real-time PCR technology. Validated on whole blood, serum, tissue from sheep, goats, other livestock. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Mycoplasma capricolum subsp. capripneumoniae (Mccp) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Mycoplasma capricolum subsp. capripneumoniae (Mccp) Real-time PCR Kit provides a fast, accurate and reliable method for detecting Mccp nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Peste des Petits Ruminants Virus (PPRV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Peste des Petits Ruminants Virus (PPRV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting PPRV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

Horses

6 kits

Respiratory outbreaks, venereal and breeding-certification pathogens, and the vector-borne diseases that shut down equine movement.

AICON-BIO® Equine Arteritis Virus (EAV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Equine Arteritis Virus (EAV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting Equine Viral Arteritis (EAV) nucleic acid using real-time PCR technology. Validated on nasopharyngeal swab, whole blood, semen from horses. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Equine Influenza (H3N8) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Equine Influenza (H3N8) Real-time PCR Kit provides a fast, accurate and reliable method for detecting Equine Influenza (H3N8) nucleic acid using real-time PCR technology. Validated on nasal swab, nasopharyngeal swab from horses. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Equine Influenza (H7N7) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Equine Influenza (H7N7) Real-time PCR Kit provides a fast, accurate and reliable method for detecting Equine Influenza (H7N7) nucleic acid using real-time PCR technology. Validated on nasal swab, nasopharyngeal swab from horses. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Taylorella equigenitalis Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Taylorella equigenitalis Real-time PCR Kit provides a fast, accurate and reliable method for detecting Taylorella equigenitalis nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

Pigs

8 kits

The enteric, respiratory and haemorrhagic pathogens that drive loss in intensive pig production — including full African and classical swine fever differentiation.

AICON-BIO® African Swine Fever Virus (ASFV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® African Swine Fever Virus (ASFV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting ASFV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Classical Swine Fever Virus (CSFV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Classical Swine Fever Virus (CSFV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting CSFV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Pasteurella multocida Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Pasteurella multocida Real-time PCR Kit provides a fast, accurate and reliable method for detecting Pasteurella multocida nucleic acid using real-time PCR technology. Validated on tracheal secretion swab, lung tissue from pigs, cattle, poultry. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Porcine Epidemic Diarrhea Virus (PEDV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Porcine Epidemic Diarrhea Virus (PEDV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting PEDV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® PRRSV North American Genotype Real-time PCR Kit

$338.00$672.00

The AICON-BIO® PRRSV North American Genotype Real-time PCR Kit provides a fast, accurate and reliable method for detecting PRRSV type 2 (North American genotype) nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Swine Influenza Virus (SIV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Swine Influenza Virus (SIV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting swine influenza virus nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Transmissible Gastroenteritis Virus (TGEV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Transmissible Gastroenteritis Virus (TGEV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting TGEV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

Poultry

8 kits

Avian influenza subtyping, notifiable disease confirmation and the breeder-flock testing that eradication programmes depend on.

AICON-BIO® Avian Infectious Bronchitis Virus (IBV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Avian Infectious Bronchitis Virus (IBV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting IBV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Avian Influenza Virus H5 Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Avian Influenza Virus H5 Real-time PCR Kit provides a fast, accurate and reliable method for detecting avian influenza virus subtype H5 nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Avian Influenza Virus H9 Real-time PCR Kit

$338.00$672.00

The AICOH-BIO® Avian Influenza H5 Real-time PCR Kit provides specific and sensitive detection of the AIV-H5 subtype using one-step TaqMan qPCR. Freeze-dried reagents ensure ultimate stability, simplifying transport and large-scale poultry diagnostics.

AICON-BIO® Avian Influenza Virus N9 Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Avian Influenza Virus N9 Real-time PCR Kit provides a fast, accurate and reliable method for detecting Avian Influenza Virus N9 nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Avian Leukosis Virus Subgroup J (ALV-J) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Avian Leukosis Virus Subgroup J (ALV-J) Real-time PCR Kit provides a fast, accurate and reliable method for detecting ALV-J nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Infectious Laryngotracheitis Virus (ILTV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Avian Infectious Laryngotracheitis Virus (ILTV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting ILTV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Newcastle Disease Virus (NDV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Newcastle Disease Virus (NDV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting NDV nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

Dogs & cats

11 kits

Clinic and shelter diagnostics: the core infectious causes of gastroenteritis, respiratory disease and vector-borne illness in small animals.

AICON-BIO® Canine Parvovirus (CPV) Real-time PCR Kit

$338.00$672.00

286

The AICON-BIO® Canine Parvovirus (CPV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting canine parvovirus nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Borrelia burgdorferi Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Borrelia burgdorferi Real-time PCR Kit provides a fast, accurate and reliable method for detecting Borrelia burgdorferi nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Canine Adenovirus Type 1 (CAV-1) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Canine Adenovirus Type 1 (CAV-1) Real-time PCR Kit provides a fast, accurate and reliable method for detecting canine adenovirus type 1 nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Canine Coronavirus (CCoV) Real-time PCR Kit

$304.20$604.80

The AICON-BIO® Canine Coronavirus (CCoV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting canine coronavirus nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Parvovirus (Canine & Feline) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Parvovirus (Canine & Feline) Real-time PCR Kit provides a fast, accurate and reliable method for detecting parvovirus nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO® Rabies Virus (RV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Rabies Virus (RV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting rabies virus nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO®Canine Adenovirus Type 1 (CAV-2) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Canine Adenovirus Type 2 (CAV-2) Real-time PCR Kit provides a fast, accurate and reliable method for detecting canine adenovirus type 2 nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

AICON-BIO®Canine Distemper Virus (CDV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Canine Distemper Virus (CDV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting canine distemper virus nucleic acid using real-time PCR technology. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain, and contains primers, probes, enzyme and dNTPs in a single tube. Available in 48T and 96T.

Multi-species & zoonotic

8 kits

Pathogens that cross species lines — tick-borne parasites, food-safety organisms and the zoonoses that need One Health surveillance.

AICON-BIO® Anaplasma spp. Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Anaplasma spp. Real-time PCR Kit provides a fast, accurate and reliable method for detecting Anaplasma spp. nucleic acid using real-time PCR technology. Validated on whole blood, spleen, tissue from cattle, sheep, dogs. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Babesia spp. Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Babesia spp. Real-time PCR Kit provides a fast, accurate and reliable method for detecting Babesia spp. nucleic acid using real-time PCR technology. Validated on whole blood, tissue, serum from cattle, sheep, horses. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Clostridium perfringens Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Clostridium perfringens Real-time PCR Kit provides a fast, accurate and reliable method for detecting Clostridium perfringens nucleic acid using real-time PCR technology. Validated on faeces, intestinal contents, tissue from pigs, cattle, sheep. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Corynebacterium spp. Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Corynebacterium spp. Real-time PCR Kit provides a fast, accurate and reliable method for detecting Corynebacterium spp. nucleic acid using real-time PCR technology. Validated on tissue, whole blood, serum from livestock, poultry. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Listeria monocytogenes Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Listeria monocytogenes Real-time PCR Kit provides a fast, accurate and reliable method for detecting Listeria monocytogenes nucleic acid using real-time PCR technology. Validated on tissue, whole blood, serum from livestock, poultry. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Rift Valley Fever Virus (RVFV) Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Rift Valley Fever Virus (RVFV) Real-time PCR Kit provides a fast, accurate and reliable method for detecting Rift Valley Fever (RVFV) nucleic acid using real-time PCR technology. Validated on whole blood, tissue, organs from cattle, sheep, goats. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Salmonella Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Salmonella Real-time PCR Kit provides a fast, accurate and reliable method for detecting Salmonella nucleic acid using real-time PCR technology. Validated on cloacal swab, faeces, caecal contents from poultry, pigs, cattle. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.

AICON-BIO® Toxoplasma gondii Real-time PCR Kit

$338.00$672.00

The AICON-BIO® Toxoplasma gondii PCR Kit is a lyophilized, one-step real-time PCR assay for the qualitative detection of T. gondii nucleic acid in whole blood, serum, brain, skeletal muscle, lymph node, liver, spleen, foetal tissue and placenta samples from sheep, goats, cats, pigs and multi-species. Supplied in 48T and 96T, it runs on any real-time PCR instrument and returns a result in about 75 minutes.

Why this kit

Why laboratories choose this Toxoplasma gondii PCR kit


AmbientNo cold chain to runLyophilized reaction tubes ship and handle at ambient temperature — no dry ice, no thaw cycles.
~75 minSample-to-answerA single 45-cycle FAM programme; results read automatically by the instrument software.
2Pipetting stepsReconstitute, add 5 µL template, run. Primers, probe, enzyme and dNTPs are already in the tube.
OpenAny qPCR platformABI, Bio-Rad, Roche, QIAGEN, Agilent and others — no proprietary instrument required.

Intended use

What the Toxoplasma gondii PCR Kit detects


In vitro qualitative detection of Toxoplasma gondii nucleic acid in samples such as whole blood, serum, tissues (brain, skeletal muscle, lymph nodes, liver, spleen) and abortion materials (foetal tissues, placenta), as an aid in the diagnosis of toxoplasmosis.

Clinical context. Toxoplasmosis — a major cause of ovine and caprine abortion and a widespread zoonosis; tissue-cyst stages make serology alone unreliable for active infection.

Target species & specimens

TargetT. gondii
Animal speciesSheep, Goats, Cats, Pigs, Multi-species
Specimen typesWhole blood, Serum, Brain, Skeletal muscle, Lymph node, Liver, Spleen, Foetal tissue, Placenta
Assay noteBroad specimen range including abortion material — designed for reproductive-loss investigation.

Specifications

Technical data


Catalogue numberAIDP0017
Pack sizes48T and 96T
MethodOne-step real-time PCR, TaqMan®-style hydrolysis probe
Reagent formatLyophilized, room-temperature-stable reaction tubes
Fluorophore / channelFAM
Reaction volume25 µL
Template input5 µL extracted nucleic acid
Instrument compatibilityAll major real-time PCR platforms — ABI, Bio-Rad, Roche LightCycler, QIAGEN Rotor-Gene, Agilent, Thermo. Add reference dye where the manufacturer recommends it.
Nucleic-acid extractionAny commercial extraction kit; not supplied
Shelf life12 months
Document referenceIFU-Toxoplasma gondii-RT-V1.2
Country of originMade in USA

Kit contents

What is in the Toxoplasma gondii PCR kit box


ComponentQuantityComposition
T. gondii Real-Time PCR Lyophilized Tube8 tests/bag × 12Target-specific primers, probe, enzyme and dNTPs
Lyophilized Reconstitution Buffer450 µL/tube × 6Buffer for reconstituting the reaction tubes
Positive Control1 tubeSynthetic plasmid carrying the target sequence
Negative Control1 tubeNuclease-free control material
Instructions for Use1 copySupplied with every kit

Quantities shown are for the 96T configuration; the 48T kit contains half quantities.

Workflow

Four steps from extract to result


  1. Extract nucleic acid with any commercial extraction kit.
  2. Reconstitute one lyophilized tube with the supplied buffer, mix, spin briefly.
  3. Combine 20 µL reconstituted reagent + 5 µL template (or control) = 25 µL reaction.
  4. Run the FAM-channel cycling programme; the instrument software reads the result.

Thermal cycling programme

CyclesTemperatureTime
1 cycle50 °C10 min
1 cycle95 °C3 min
45 cycles95 °C15 sec
 60 °C (FAM read)30 sec

Set the reaction volume to 25 µL and select the FAM channel. On ABI systems leave the analysis threshold at its default value of 0.2.

Result interpretation

Reading the run


Ct valueInterpretation
Ct < 37Positive
37 ≤ Ct < 45Suspicious — retest with a fresh sample
No CtNegative
Run validity. The negative control must show no amplification and the positive control a clear amplification curve. If the negative control amplifies, suspect contamination and repeat; if the positive control fails, the run is invalid.

Sample handling

Specimen collection & storage


TissueGreen-bean-sized piece into a 1.5 mL tube with 500 µL preservation solution; grind with a disposable pestle; centrifuge 1 min; take the supernatant.
Whole bloodCollect into an EDTA anticoagulant tube.
SerumAllow the blood to clot or centrifuge at low speed; collect the supernatant.
SwabSmear the sampling site, place the tip into a 1.5 mL tube with 500 µL preservation solution; grind a few seconds; centrifuge 1 min; take the supernatant.

Storage & stability

Lyophilized reagents ship at room temperature — no cold chain required. On arrival, store the lyophilized reagents at −20 °C; liquid reagents below 4 °C. Stable until the expiry date printed on the label. Keep the tubes sealed in their aluminium pouches to prevent moisture uptake and bring them to room temperature before use. Once reconstituted, seal tightly, store at −20 °C and use within one month.

Related tests

Other kits for the same animals


See the full range in Animal Disease Testing. Disease definitions and notifiable-disease status follow WOAH, the World Organisation for Animal Health.

For Research Use Only. Not for use in diagnostic procedures. Results should be interpreted together with clinical findings, epidemiological context and other laboratory results. Performance characteristics have not been established for regulated diagnostic testing in every jurisdiction — contact info@aiconbiotech.com for the regulatory status in your market.

Custom development

Not in the catalogue? We will make it.

The 50 kits above are a starting point, not a limit. If the target you need is missing — or the format, the branding or the pack size is not what your market expects — tell us and we build it.

Custom assay development

Name the target — a pathogen, a serotype, a resistance gene, a genotype you need separated from its neighbours — and we design, screen and validate the primer/probe set, delivered in the same lyophilized single-tube format as the catalogue kits.

Multiplex & panel design

Two to four targets in one reaction, or a fixed plate panel built around a species or a syndrome. Useful where sample volume is limited or a differential list has to be cleared in a single run.

OEM & private label

Your brand on the box, the label and the instructions for use. Your catalogue numbers, your document format, your language. Minimum order quantities low enough to test a market first.

Kit configuration

48T, 96T or a size we cut for you. Reaction volume, tube format, control material and the number of reactions per lyophilized tube can all be specified.

Lyophilization service

We freeze-dry your own master mix into ambient-stable single-tube reactions — the same process behind every kit on this page.

Documentation & support

Instructions for use, certificates of analysis, batch records and validation summaries prepared to your registration requirements, with technical support through set-up.

How it works

From enquiry to first batch


  1. Tell us the targetName the organism, the sample matrix and the animal species, plus any sequences or strains you need covered or excluded. Reference material helps but is not always required.
  2. We design and pilotWe come back with a design proposal and a timeline, screen candidate primer/probe sets, and send you pilot kits to run on your own samples before any production batch is committed.
  3. Production and documentationYour batch ships with instructions for use, certificate of analysis and the validation summary your registration requires — under your label if you want it.

Ordering

Distributors and bulk orders


Buy online
Every kit on this page can be ordered directly, in 48T or 96T. Distributor pricing, bulk configurations and OEM labelling are quoted on request — send us the catalogue numbers and quantities and we will come back with pricing, lead time and shipping options.
Aicon Biotech Inc
10130 SW Nimbus Ave
Tigard, Oregon 97223, United States

Tel +1 503-430-5188
info@aiconbiotech.com
Disease definitions: WOAH
For Research Use Only. Not for use in diagnostic procedures. Results should be interpreted together with clinical findings, epidemiological context and other laboratory results. Performance characteristics have not been established for regulated diagnostic testing in every jurisdiction — contact info@aiconbiotech.com for the regulatory status of a given product in your market.