AICON-BIO® Brucella melitensis Real-time PCR Kit
$338.00 – $672.00
The AICON-BIO® Brucella melitensis Real-time PCR Kit provides a fast, accurate and reliable method for detecting Brucella melitensis nucleic acid using real-time PCR technology. Validated on whole blood, serum, tissue from sheep, goats, other livestock. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.
The AICON-BIO® Brucella melitensis PCR Kit is a lyophilized, one-step real-time PCR assay
for the qualitative detection of Brucella melitensis nucleic acid in whole blood, serum, tissue, swab from
sheep, goats, other livestock. Supplied in 48T and 96T, it runs on any real-time PCR instrument and
returns a result in about 75 minutes.
Why this kit
Why laboratories choose this Brucella melitensis PCR kit
Lyophilized reaction tubes ship and handle at ambient temperature — no dry ice, no thaw cycles.
A single 45-cycle FAM programme; results read automatically by the instrument software.
Reconstitute, add 5 µL template, run. Primers, probe, enzyme and dNTPs are already in the tube.
ABI, Bio-Rad, Roche, QIAGEN, Agilent and others — no proprietary instrument required.
Intended use
What the Brucella melitensis PCR Kit detects
Real-time fluorescent PCR for the in vitro qualitative detection of Brucella melitensis (Bm) nucleic acid in clinical samples from sheep, goats and other animals, providing a molecular basis for the clinical diagnosis of B. melitensis infection.
Target species & specimens
| Target | Brucella melitensis |
|---|---|
| Animal species | Sheep, Goats, Other livestock |
| Specimen types | Whole blood, Serum, Tissue, Swab |
| Assay note | Species-specific for Brucella melitensis (Bm), distinguishing it from B. abortus. |
Specifications
Technical data
| Catalogue number | AIDB0031 |
|---|---|
| Pack sizes | 48T and 96T |
| Method | One-step real-time PCR, TaqMan®-style hydrolysis probe |
| Reagent format | Lyophilized, room-temperature-stable reaction tubes |
| Fluorophore / channel | FAM |
| Reaction volume | 25 µL |
| Template input | 5 µL extracted nucleic acid |
| Instrument compatibility | All major real-time PCR platforms — ABI, Bio-Rad, Roche LightCycler, QIAGEN Rotor-Gene, Agilent, Thermo. Add reference dye where the manufacturer recommends it. |
| Nucleic-acid extraction | Any commercial extraction kit; not supplied |
| Shelf life | 12 months |
| Document reference | IFU-Brucella melitensis-RT-V1.0 |
| Country of origin | Made in USA |
Kit contents
What is in the Brucella melitensis PCR kit box
| Component | Quantity | Composition |
|---|---|---|
| Brucella melitensis Real-Time PCR Lyophilized Tube | 8 tests/bag × 12 | Target-specific primers, probe, enzyme and dNTPs |
| Lyophilized Reconstitution Buffer | 450 µL/tube × 6 | Buffer for reconstituting the reaction tubes |
| Positive Control | 1 tube | Synthetic plasmid carrying the target sequence |
| Negative Control | 1 tube | Nuclease-free control material |
| Instructions for Use | 1 copy | Supplied with every kit |
Quantities shown are for the 96T configuration; the 48T kit contains half quantities.
Workflow
Four steps from extract to result
- Extract nucleic acid with any commercial extraction kit.
- Reconstitute one lyophilized tube with the supplied buffer, mix, spin briefly.
- Combine 20 µL reconstituted reagent + 5 µL template (or control) = 25 µL reaction.
- Run the FAM-channel cycling programme; the instrument software reads the result.
Thermal cycling programme
| Cycles | Temperature | Time |
|---|---|---|
| 1 cycle | 50 °C | 10 min |
| 1 cycle | 95 °C | 3 min |
| 45 cycles | 95 °C | 15 sec |
| 60 °C (FAM read) | 30 sec |
Set the reaction volume to 25 µL and select the FAM channel. On ABI systems leave the
analysis threshold at its default value of 0.2.
Result interpretation
Reading the run
| Ct value | Interpretation |
|---|---|
| Ct < 37 | Positive |
| 37 ≤ Ct < 45 | Suspicious — retest with a fresh sample |
| No Ct | Negative |
no amplification and the positive control a clear amplification curve. If the negative control
amplifies, suspect contamination and repeat; if the positive control fails, the run is invalid.
Sample handling
Specimen collection & storage
| Tissue | Green-bean-sized piece into a 1.5 mL tube with 500 µL preservation solution; grind with a disposable pestle; centrifuge 1 min; take the supernatant. |
|---|---|
| Whole blood | Collect into an EDTA anticoagulant tube. |
| Serum | Allow the blood to clot or centrifuge at low speed; collect the supernatant. |
| Swab | Smear the sampling site, place the tip into a 1.5 mL tube with 500 µL preservation solution; grind a few seconds; centrifuge 1 min; take the supernatant. |
Storage & stability
Lyophilized reagents ship at room temperature — no cold chain required. On arrival, store the lyophilized reagents at −20 °C; liquid reagents below 4 °C. Stable until the expiry date printed on the label. Keep the tubes sealed in their aluminium pouches to prevent moisture uptake and bring them to room temperature before use. Once reconstituted, seal tightly, store at −20 °C and use within one month.
Related tests
Other kits for the same animals
See the full range in Animal Disease Testing. Disease definitions
and notifiable-disease status follow
WOAH, the World Organisation for Animal Health.
Results should be interpreted together with clinical findings, epidemiological context and other
laboratory results. Performance characteristics have not been established for regulated
diagnostic testing in every jurisdiction — contact
info@aiconbiotech.com for the regulatory status in
your market.
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| size |
48T ,96T |
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