Common name: flagella staining solution
For the staining of bacterial flagella.
Bacterial flagella is a moving organ of bacteria. It must be observed by electron microscopy or by special staining to make the flagella thicken and stained. It can be seen under ordinary microscope. The principle of this dyeing is the latter. Through flagellar staining, the morphology, quantity and flagellar distribution of flagella in the cell body can be observed. The number of flagella and the distribution position on the cells are one of the important basis for identifying bacteria.
|Reagent composition||Main ingredient|
|Sodium chloride solution
|Tannic acid solution (solution B)||Tannic acid|
|Magenta alcohol solution
|Alkaline magenta , alcohol|
Storage conditions and expiration date
Store at 2 °C ~ 8 °C, valid for 24 months. It is stored at 2°C ~8°C after the opening period, and the validity period is 6 months.
Preparation of smear: Method 1. Inoculate the strain in the broth culture tube, incubate at 37 ° C for 24 hours, centrifuge, pour off the supernatant, and place a ring on the end of the slide with a loop of the inoculating ring. The slide was tilted so that the bacterial solution naturally flowed to the other end of the slide and allowed to dry naturally at room temperature. Method 2: Inoculate the strain into the bottom of the inclined agar medium (previously add liquid medium or sterile physiological saline at the bottom), and after incubation (8~16) hours, take the lawn on the inclined surface and suspend it in sterile. In the water, make it slightly turbid. A loop of the above-mentioned bacterial solution was taken with an inoculating ring, placed at one end of the slide, and the slide was poured, so that the bacterial liquid naturally flowed to the other end of the slide, and it was naturally dried at room temperature.
1. Preparation of working fluid: Before use, mix according to the ratios of liquid A, liquid B, and liquid C, and put them in a filter bottle, and mix and let stand for 2 hours before use.
2. Drip the working solution onto the smear and dye for about 10 ~15 minutes;
3. Slightly tilt the slide and rinse it off with distilled water;
4, the slides stand upright to allow the water to run out, naturally dry, microscopic examination.
Explanation of test results
The cells and flagella are red in varying degrees.
Limitations of inspection methods
It is only used for morphological staining observations.
1. The success of flagella dyeing has a very close relationship with the cleanliness of the slide.
2. After the working fluid is dyed, the water washing strength should not be too large, otherwise the flagella and bacteria will be washed away.
3. This reagent should be used by professionals and the interpretation of the results.
4. Read the instruction manual in detail before use, use it within the validity period, and do personal hygiene protection.
5. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
6. The production date, production batch number and expiration date are shown in the outer packaging.