Product Descriptions: RPMI 1640 Medium
Using RPMI 1640
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. This product is made of cell culture medium of powder that needs to supplement sodium bicarbonate, adjust pH value and filter.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Contents & storage
Storage conditions: 2-8° C
Shelf life: 60 months from date of manufacture