AICON-BIO® Avian Influenza Virus H7 (H7-HPAI) Real-time PCR Kit

Why this kit

Built for field-realistic veterinary laboratories

Intended use

What the Avian Influenza Virus H7 (H7-HPAI) kit detects

The AICON-BIO® Avian Influenza Virus H7 Real-time PCR Kit uses real-time PCR technology and a premixed freeze-drying method to detect avian influenza virus subtype H7 nucleic acid. H7 is one of only two haemagglutinin subtypes able to acquire a highly pathogenic phenotype; low-pathogenic H7 viruses circulating in poultry can mutate into highly pathogenic forms that kill most of a flock within 48 hours.

H7 infections are WOAH-notifiable, and several H7 subtypes — H7N9 in particular — have caused serious human disease. Early subtype identification is what allows control measures to begin before the virus becomes highly pathogenic. The Kit qPCR mix (lyophilized) contains primers, probes, enzyme and dNTPs in one tube.

Target species & specimens

Specifications

Technical data

Kit contents

What is in the box

Quantities shown are for the 96T configuration; the 48T kit contains half quantities.

Workflow

Four steps from extract to result

1. Extract nucleic acid with any commercial extraction kit.
2. Reconstitute one lyophilized tube with the supplied buffer, mix, spin briefly.
3. Combine 20 µL reconstituted reagent + 5 µL template (or control) = 25 µL reaction.
4. Run the FAM-channel cycling programme; the instrument software reads the result.

Thermal cycling programme

Set the reaction volume to 25 µL and select the FAM channel. On ABI systems leave the
analysis threshold at its default value of 0.2.

Result interpretation

Reading the run

Sample handling

Specimen collection & storage

Storage & stability

Lyophilized reagents ship at room temperature — no cold chain required. On arrival, store the lyophilized reagents at −20 °C; liquid reagents below 4 °C. Stable until the expiry date printed on the label. Keep the tubes sealed in their aluminium pouches to prevent moisture uptake and bring them to room temperature before use. Once reconstituted, seal tightly, store at −20 °C and use within one month.