AICON-BIO® Anaplasma spp. Real-time PCR Kit
$338.00 – $672.00
The AICON-BIO® Anaplasma spp. Real-time PCR Kit provides a fast, accurate and reliable method for detecting Anaplasma spp. nucleic acid using real-time PCR technology. Validated on whole blood, spleen, tissue from cattle, sheep, dogs. The lyophilized one-step qPCR mix stays stable during transport and storage without a cold chain. Available in 48T and 96T.
The AICON-BIO® Anaplasma PCR Kit is a lyophilized, one-step real-time PCR assay for the qualitative detection of Anaplasma spp. nucleic acid in whole blood, spleen and tissue samples from cattle, sheep, dogs and horses. Supplied in 48T and 96T, it runs on any real-time PCR instrument and returns a result in about 75 minutes.
Why this kit
Why laboratories choose this Anaplasma PCR kit
Intended use
What the Anaplasma PCR Kit detects
Real-time fluorescent PCR for the in vitro qualitative detection of Anaplasma spp. nucleic acid in blood, spleen and other samples from cattle, sheep, dogs and horses. Intended for molecular detection and differential diagnosis of animal anaplasmosis, including bovine Anaplasma marginale, ovine Anaplasma, canine Anaplasma platys and Anaplasma phagocytophilum.
Target species & specimens
| Target | Anaplasma spp. |
|---|---|
| Animal species | Cattle, Sheep, Dogs, Horses |
| Specimen types | Whole blood, Spleen, Tissue |
| Assay note | Detects bovine Anaplasma marginale, ovine Anaplasma, canine Anaplasma platys and Anaplasma phagocytophilum. |
Specifications
Technical data
| Catalogue number | AIAS0011 |
|---|---|
| Pack sizes | 48T and 96T |
| Method | One-step real-time PCR, TaqMan®-style hydrolysis probe |
| Reagent format | Lyophilized, room-temperature-stable reaction tubes |
| Fluorophore / channel | FAM |
| Reaction volume | 25 µL |
| Template input | 5 µL extracted nucleic acid |
| Instrument compatibility | All major real-time PCR platforms — ABI, Bio-Rad, Roche LightCycler, QIAGEN Rotor-Gene, Agilent, Thermo. Add reference dye where the manufacturer recommends it. |
| Nucleic-acid extraction | Any commercial extraction kit; not supplied |
| Shelf life | 12 months |
| Document reference | IFU-Anaplasma spp.-RT-V1.1 |
| Country of origin | Made in USA |
Kit contents
What is in the Anaplasma PCR kit box
| Component | Quantity | Composition |
|---|---|---|
| Anaplasma spp. Real-Time PCR Lyophilized Tube | 8 tests/bag × 12 | Target-specific primers, probe, enzyme and dNTPs |
| Lyophilized Reconstitution Buffer | 450 µL/tube × 6 | Buffer for reconstituting the reaction tubes |
| Positive Control | 1 tube | Synthetic plasmid carrying the target sequence |
| Negative Control | 1 tube | Nuclease-free control material |
| Instructions for Use | 1 copy | Supplied with every kit |
Quantities shown are for the 96T configuration; the 48T kit contains half quantities.
Workflow
Four steps from extract to result
- Extract nucleic acid with any commercial extraction kit.
- Reconstitute one lyophilized tube with the supplied buffer, mix, spin briefly.
- Combine 20 µL reconstituted reagent + 5 µL template (or control) = 25 µL reaction.
- Run the FAM-channel cycling programme; the instrument software reads the result.
Thermal cycling programme
| Cycles | Temperature | Time |
|---|---|---|
| 1 cycle | 50 °C | 10 min |
| 1 cycle | 95 °C | 3 min |
| 45 cycles | 95 °C | 15 sec |
| 60 °C (FAM read) | 30 sec |
Set the reaction volume to 25 µL and select the FAM channel. On ABI systems leave the analysis threshold at its default value of 0.2.
Result interpretation
Reading the run
| Ct value | Interpretation |
|---|---|
| Ct < 37 | Positive |
| 37 ≤ Ct < 45 | Suspicious — retest with a fresh sample |
| No Ct | Negative |
Sample handling
Specimen collection & storage
| Tissue | Green-bean-sized piece into a 1.5 mL tube with 500 µL preservation solution; grind with a disposable pestle; centrifuge 1 min; take the supernatant. |
|---|---|
| Whole blood | Collect into an EDTA anticoagulant tube. |
| Serum | Allow the blood to clot or centrifuge at low speed; collect the supernatant. |
| Swab | Smear the sampling site, place the tip into a 1.5 mL tube with 500 µL preservation solution; grind a few seconds; centrifuge 1 min; take the supernatant. |
Storage & stability
Lyophilized reagents ship at room temperature — no cold chain required. On arrival, store the lyophilized reagents at −20 °C; liquid reagents below 4 °C. Stable until the expiry date printed on the label. Keep the tubes sealed in their aluminium pouches to prevent moisture uptake and bring them to room temperature before use. Once reconstituted, seal tightly, store at −20 °C and use within one month.
Related tests
Other kits for the same animals
See the full range in Animal Disease Testing. Disease definitions and notifiable-disease status follow WOAH, the World Organisation for Animal Health.
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Size |
48T ,96T |
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