Gram Stain(100ml)

Gram Stain(100ml)

  • Model: AI-VDB01
  • Price:


    Note: Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.

    Product Description

    Product name

    Common name: Gram stain

    English name: Gram Stain

    Package Specifications

    Single bottle (box) Packing specification: 100ml

    Set (box) Packing specification: 4×100ml

    Expected usage

    Smear staining for bacteria or fungi.

    Test principle

    Gram staining is a method of differential staining widely used in bacteriology. It can be used for specimen smear or colony smear. After staining, the bacteria form a sharp contrast with the environment, and the morphology, arrangement and some of the bacteria can be clearly observed. These structural features are used for clinical classification and identification. The staining results divided the bacteria into two major categories: Gram-positive bacteria (purple) and Gram-negative bacteria (red). Gram-positive bacteria have thicker cell walls, more peptidoglycan network layers and dense cross-linking. After Gram staining, crystal violet and iodine complexes can be firmly retained in the wall, which is not easily removed by decolorizing liquid. It is still purple; Gram-negative bacteria have a thin cell wall, a high lipid content in the outer membrane layer, a thin peptidoglycan layer and poor cross-linking. After the decolorizing agent, the lipid-based outer membrane rapidly Dissolved, thin and loose peptidoglycan network can not block the dissolution of crystal violet and iodine complex, so it is still colorless after decolorization by decolorizing solution, and then counterstained with sand yellow solution, the gram-negative bacteria are red.

    Main components

    Reagent composition

    Main ingredient

    Crystal Violet

    Gentian violet , Ethanol

    Iodine solution

    Potassium iodide , iodine



    Safranin Solution

    Safrani , Ethanol,

    Alkaline magenta

    Storage conditions and expiration date

    5 ° C ~ 30 ° C protected from light, valid for 24 months.

    The original packaging does not open the dyeing liquid for a period of 24 months. The opened dyeing solution is recommended to be used within 6 months after opening. After each use, the cap should be tightened in time to avoid evaporation or deterioration.

    Sample requirements

    Care must be taken when smearing, and the smear is thin and uniform. The prepared smear should be naturally dry. When fixing with heat, the fixing temperature should not be too high. The back of the slide should not be hot to the back of the hand. Otherwise, the bacterial morphology may affect the observation.

    Testing method

    1. Add gentian violet liquid for 10 seconds, wash with water, dry;

    2. Dyeing with iodine solution for 10 seconds, washing with water, drying;

    3. Decolorize the solution for 10-20 seconds, wash with water, dry;

    4. Finally, add the yellow solution of the solution to the dyeing solution for 10 seconds, and wash it with water;

    5. Wait until dry, microscopic examination.

    Explanation of test results

    Gram-positive bacteria are purple; Gram-negative bacteria are red.

    Limitations of inspection methods

    There is a possibility of false negatives when using smears of old specimens.


    1. The dyeing time should be adjusted according to what kind of specimen, smear thickness, etc.; when dyeing gynecological leucorrhea smear, the dyeing time should be slightly extended (not less than 10 seconds) to obtain better dyeing effect.

    2. If the smear is too thick, the decolorization time is insufficient, and the long time of gentian violet staining may lead to false positive results; excessive heat fixation of bacterial smears, too long bacterial culture time, excessive discoloration, etc. may lead to false negative results. .

    3. When the room temperature is too low in winter, the dyeing time should be extended appropriately.

    4. Try to avoid high and low temperature environment and direct sunlight when storing reagents.

    5. After the reagents are used up, please cover them quickly to avoid evaporation.

    6. This product should be used by professionals and the interpretation of the results.

    7. Read the instruction manual carefully before use, use it within the validity period, and do personal protection.

    8. Dispose of waste according to the requirements of the hospital or environmental protection department after use.

    9. The production date, production batch number and expiration date are shown in the outer packaging.


    • Model: AI-VDB01
    • 998 Units in Stock


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    Shipping info

    $50.00 to United States

    Contact us to order


    This product was added to our catalog on Monday 04 March, 2019.