Unable to sell in the United States
AICON™ Fetal Bovine Serum (FBS), Uruguay origin, is filtered using 100 nm (0.1 µm) pore size-rated filters and processed using true pool technology to ensure consistency in the quality of the serum. AICON™ FBS Uruguay Origin is compliant with current good manufacturing practices (cGMP). AICON™ FBS is widely used by professionals requiring an extensive biochemical profile and full traceability back to the original source.
Key features of AICON™ FBS, Uruguay origin:
• Sourced in Uruguay
• Low in antibodies and high in growth factors
• Complete traceability back to original source
• Virus panel testing according to 9 CFR 113.53
• Produced using true pool technology for uniformity and consistency from bottle to bottle
Specifications
AICON™ FBS is filtered through three sequential 100 nm (0.1 μm) pore size-rated filters. Before dispensing, each lot of serum is pooled to ensure uniformity and consistency between bottles (true pool technology).
AICON™ FBS is assayed for gamma globulin, alkaline phosphatase, lactate dehydrogenase, glutamic pyruvic
transaminase (SBPT), glutamic oxaloacetic transaminase (SGOT), pH, total protein, albumin, blood urea nitrogen, creatinine, total bilirubin, sodium, potassium, calcium, chloride, inorganic phosphorus, osmolality, iron, total iron binding capacity (TIBC), percent iron saturation, glucose, and IgG. Included assays are subject to change without notice.
Storage and handling
Sera should be stored at -10°C or lower. Once thawed, sera should be stored at 2°C to 8°C. To maintain quality, a conservative storage recommendation for sera at this temperature is no longer than six weeks. If the serum needs to be stored longer than six weeks after opening, it is recommended to aliquot the serum into convenient volumes and refreeze.
Thawing
Remove serum from -10°C (or lower) storage and place in a refrigerator overnight at 2°C to 8°C. Transfer the serum to a 37°C water bath, agitate periodically to mix the solutes concentrated at the bottom of the container. Do not hold the serum at 37°C any longer than necessary after thawing. Thawing serum in a bath above 40°C without mixing can denature the concentrated proteins in the bottom of the container and precipitates might form in the bottle. Thawing serum at higher temperatures is not recommended.
Alternatively, bottles can be placed directly from -10°C (or lower) storage into a 37°C water bath. Bottles should be agitated to enhance mixing and thawing. Turbidity and flocculent material might be present after thawing or after prolonged storage. Experience indicates that regardless of the method used to thaw
serum, it is critical that it is mixed during the thawing process to prevent the formation of gradients and subsequent precipitation. Because of differences in thawing rates of different components, serum will form a gradient if it is not mixed as it thaws. If serum is allowed to remain in such a gradient state, precipitation is
likely to occur. Handle bottles that have been stored in freezer carefully. Avoid large temperature shifts and protect the serum from exposure to light. Wear protective clothes and equipment. Storage requirements are listed on the product label.
General culture recommendations
We recommend supplementation of classical media at a range between 5% and 10% FBS to support culture of a wide variety of cell lines and applications.
Quality control testing
Endotoxin ≤ 25 EU/mL
(Limulus amebocyte lysate gel clot assay
Hemoglobin (spectrophotometric) < 25 mg/dL
Sterility testing (current USP) No growth
Virus Testing (9 CFR 113.53)
Fluorescent Antibody
Rabies Not detected
Reovirus Not detected
Bluetongue Not detected
Bovine parvovirus Not detected
Bovine adenovirus Not detected
Bovine viral diarrhea virus Tested and reported
Bovine respiratory syncytial virus Not detected
Hemadsorbing agents (e.g., PI3) Not detected
Cytopathogenic agents (e.g., IBR) Not detected
Mycoplasma
Hoechst DNA stain Not detected
Large volume, direct culture Not detected
